Product
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Protino® Ni-IDA Resin
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Company
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MACHEREY-NAGEL
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Catalog No.
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745210.5 / .30 / .120 / .600
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package
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5 30 120 and 600 g
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Feuture
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Protino® Ni-IDA best choice for higher yields of His-tag proteins • Chelating group IDA (iminodiacetic acid) three protein binding sites • High protein yield / recovery even from diluted samples • High protein concentration • High binding specificity less unspecific binding of contaminating proteins compared to other common IMAC matrices • Dry resin – storage at room temperature Protino® Ni-IDA Resin • Dry bulk material • Rigid support silica matrix, compatible with high pressure and high flow rates does not shrink or swell – highest flexibility • Batch procedure gravity-flow column chromatography FPLC™ applications • Empty Protino® columns available for use with Protino® Ni-IDA Resin • Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin |
Application
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• Purification of polyhistidine-tagged proteins • Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications |
Technology
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IMAC (immobilized metal ion affinity chromatography)
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Format
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bulk resin
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Binding capacity
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20 mg/g
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Amount of resin per column
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-
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Max. pressure
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145 psi (10 bar)
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Matrix
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Macroporous silica
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Chelating ligand
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IDA (iminodiacetic acid)
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Physical form
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Dry matrix, precharged with Ni2+ |
Manual
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สินค้าที่เกี่ยวข้อง
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