RNA-direct™ SYBR® Green Realtime PCR Master Mix

RNA-direct™ SYBR® Green Realtime PCR Master Mix

TYB-QRT-201   

This product is a 2 × Master Mix for "1-step real-time PCR" using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8 (1). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for "1-step real-time PCR", including reverse transcription and PCR steps. RNA-direct™ Realtime PCR Master Mix is applicable for TaqMan® assay or hybridization probe assay, in combination with each probe. RNA-direct™ SYBR Green® Realtime PCR Master Mixcan be applied to an intercalation assay with SYBR® Green I.

1 Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
2 Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
3 Can be used in systems using passive reference (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.).
4 Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible amplification.

0.5 mL x 5 / 100 reactions

One-step qPCR Master Mix Intercalation assay with SYBR® Green I

TOYOBO