Product
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RNA-direct™ Realtime PCR Master Mix
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Catalog No.
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TYB-QRT-101
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Description
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This product is a 2 × Master Mix for "1-step real-time PCR" using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8 (1). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for "1-step real-time PCR", including reverse transcription and PCR steps. RNA-direct™ Realtime PCR Master Mix is applicable for TaqMan® assay or hybridization probe assay, in combination with each probe. RNA-direct™ SYBR Green® Realtime PCR Master Mixcan be applied to an intercalation assay with SYBR® Green I.
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Feature
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1 Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination. 2 Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.). 3 Can be used in systems using passive reference (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.). 4 Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible amplification. |
Volumn Unit and Reaction
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0.5 mL x 5 / 100 reactions |
Application
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One-step qPCR Master Mix
TaqMan® assays or hybridization probe assays
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Company
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TOYOBO
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Manual
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สินค้าที่เกี่ยวข้อง
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