RT-PCR Quick Master Mix

RT-PCR Quick Master Mix

TYB-PCR-311

RT-PCR Quick Master Mix provides a 2 x Master Mix for RT-PCR using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8 (1). Tth DNA polymerase exhibits reverse transcriptase activity in addition to DNA polymerase activity in the presence of Mn2+ ions. Therefore, this system enables "one-step RT-PCR" including reverse transcription and PCR steps. This kit is suitable for high-throughput RT-PCR, and decreases contamination risks.

1 This kit enables one-step RT-PCR including reverse transcription and PCR steps. This kit is suitable for high-throughput RT-PCR and decreases contamination risks.
2 Compared with normal reverse transcriptases from retroviruses (e.g., M-MLV reverse transcriptase), Tth DNA polymerase can work at a higher temperature. Therefore, this kit has the advantage of amplifying targets with complex conformations and GC-rich content.
3 Hot-start technology using anti-Tth DNA polymerase antibodies enables highly efficient and specific amplification.

50 reactions

One-step RT-PCR

TOYOBO