KOD Dash

Product	KOD Dash
Catalog No.	TYB-LDP-101
Description	KOD Dash is a highly efficient DNA polymerase mixture developed based on the Barns' method(1). This method uses a DNA polymerase which lacked a 3'→5' exonuclease (proofreading) activity and a small amount of an archaeal DNA polymerase with proofreading activity. In the reagent, the 3'→5' exonuclease activity-deficient mutant of KOD DNA polymerase(2) and KOD DNA polymerase are used. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of a polymerase reaction, PCR with this mixed enzyme solution enables highly efficient amplification. KOD Dash generates dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA cloning method.
Feature	1 Effective for the amplification of various targets from a small starting template amount. 2 Shows greater elongation velocity than Taq DNA polymerase (2 folds) and Pfu DNA polymerase (6 folds) due to the intrinsic property of KOD DNA polymerase. 3 The PCR error ratio of this enzyme mixture is approximately 3 to 4 times less than that of Taq DNA polymerase.
Volumn Unit and Reaction	250 U / 200 reactions
Application	High Speed & Efficient DNA Polymerase
Company	TOYOBO
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