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KOD -Plus- Neo
เพิ่มเมื่อ: 2016-12-23 14:07:37.0
รายละเอียด:
High fidelity PCR Fast and efficient PCR
High fidelity PCR Fast and efficient PCR
- Catalog No.TYB-KOD-401
- DescriptionKOD -Plus- Neo is based on a DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1(1) (2). This polymerase exhibits excellent PCR fidelity because of its efficient 3'→5' exonuclease activity (proof reading activity). This product contains a unique "elongation enhancer" that suppresses the "plateau effect" produced by conventional PCR. Therefore, this reagent exhibits greater amplification efficiency and elongation capability compared to the previous version of KOD -Plus- (Code No. KOD-201). Moreover, this enzyme requires only 30 sec/kb for the PCR extension step. This facilitates the long range PCR. This enzyme contains two anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR(3). This polymerase generates blunt-end PCR products due to 3'→5' exonuclease (proof-reading) activity.
- FeatureKOD -Plus- Neo exhibits 80-fold greater PCR fidelity than Taq DNA polymerase. 2 "Elongation enhancer" enables greater 3 amplification efficiency and elongation capability (up to 24 kb from human genomic DNA) compared to conventional PCR. 4 Requires only 30 sec/kb for the PCR extension step. 5 2-step cycle conditions can be used for amplification using ≥ 20 mer primers (melting temperatures, Tm >63ºC).
- Volumn Unit200 U / 200 reactions
- ApplicationHigh fidelity PCR Fast and efficient PCR
- CompanyTOYOBO
- ManualKOD -Plus- Neo
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Product
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KOD -Plus- Neo
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Catalog No.
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TYB-KOD-401
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Description
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KOD -Plus- Neo is based on a DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1(1) (2). This polymerase exhibits excellent PCR fidelity because of its efficient 3'→5' exonuclease activity (proof reading activity). This product contains a unique "elongation enhancer" that suppresses the "plateau effect" produced by conventional PCR. Therefore, this reagent exhibits greater amplification efficiency and elongation capability compared to the previous version of KOD -Plus- (Code No. KOD-201).
Moreover, this enzyme requires only 30 sec/kb for the PCR extension step. This facilitates the long range PCR.
This enzyme contains two anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR(3). This polymerase generates blunt-end PCR products due to 3'→5' exonuclease (proof-reading) activity.
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Feature
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KOD -Plus- Neo exhibits 80-fold greater PCR fidelity than Taq DNA polymerase. 2 "Elongation enhancer" enables greater 3 amplification efficiency and elongation capability (up to 24 kb from human genomic DNA) compared to conventional PCR. 4 Requires only 30 sec/kb for the PCR extension step. 5 2-step cycle conditions can be used for amplification using ≥ 20 mer primers (melting temperatures, Tm >63ºC). |
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Volumn Unit and Reaction
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200 U / 200 reactions |
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Application
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High fidelity PCR
Fast and efficient PCR
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Company
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TOYOBO
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Manual
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