Cy2-AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgM, ? Chain Specific

JIR-715-226-020

Cy2-AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgM, ? Chain Specific

1 mg

Cy2

Mouse

Donkey

F(ab')? Fragment

IgM, ? Chain

Polyclonal

Freeze-dried powder

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.

one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15mg/mL BSA, 0.05% Azide

Azide

F(ab')₂ fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')₂ fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of mouse IgM but not with mouse IgG or the light chains of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgM from other species.

Jackson ImmunoResearch