Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on mouse IgG and with those common to other mouse immunoglobulins. Reaction is primarily with kappa light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.
Catalog No.
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JIR-115-005-174
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Product Name
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AffiniPure Goat Anti-Mouse IgG, Light Chain* Specific (min X Bov,Gt,Hrs,Hu,Rb,Rat,Shp Ig)
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Unit
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1 mg
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Conjugated
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Unconjugated
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Target
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Mouse
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Host
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Goat
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Product Type
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Whole IgG
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Specificity
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IgG, Light Chain
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Clonality
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Polyclonal
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Physical State
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Sterile-filtered liquid
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Storage
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Store at 2-8°C under sterile conditions. Prepare working dilution fresh each day.
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Expiration date
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one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
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Purity
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Buffer
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0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
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Preservative
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Working Concentration
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Information
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Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on mouse IgG and with those common to other mouse immunoglobulins. Reaction is primarily with kappa light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species. |
Company
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Jackson ImmunoResearch
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สินค้าที่เกี่ยวข้อง
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