Alexa Fluor? 488-AffiniPure Goat Anti-Rat IgG, Light Chain* Specific (min X Bov,Gt,Hrs,Hu,Ms,Rb,Shp Ig)

JIR-112-545-175

Alexa Fluor? 488-AffiniPure Goat Anti-Rat IgG, Light Chain* Specific (min X Bov,Gt,Hrs,Hu,Ms,Rb,Shp Ig)

0.5 mg

Alexa Fluor? 488

Rat

Goat

Whole IgG

IgG, Light Chain

Polyclonal

Freeze-dried powder

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.

one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15mg/mL BSA, 0.05% Azide

Azide

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on rat IgG and with those common to other rat immunoglobulins. Reaction is primarily with kappa light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.

Jackson ImmunoResearch