Alexa Fluor? 488-AffiniPure Goat Anti-Rat IgG, F(ab')2 Fragment Specific

JIR-112-545-006

Alexa Fluor? 488-AffiniPure Goat Anti-Rat IgG, F(ab')2 Fragment Specific

1.5 mg

Alexa Fluor? 488

Rat

Goat

Whole IgG

IgG, F(ab')? Fragment

Polyclonal

Freeze-dried powder

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.

one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15mg/mL BSA, 0.05% Azide

Azide

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')₂/Fab portion of rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against the Fc portion of rat IgG or against non-immunoglobulin serum proteins. The antibody may cross-react with immunogloublins from other species.

Jackson ImmunoResearch