Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, horse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
Catalog No.
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JIR-112-185-143
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Product Name
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4nm Colloidal Gold-AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs,Rb Sr Prot) (LM Grade)
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Unit
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1 ml
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Conjugated
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4 nm Gold
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Target
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Rat
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Host
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Goat
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Product Type
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Whole IgG
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Specificity
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IgG (H+L)
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Clonality
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Polyclonal
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Physical State
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Freeze-dried powder
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Storage
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Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. After rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Use an aliquot within one day of thawing.
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Expiration date
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one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
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Purity
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Buffer
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0.01M Sodium Borate-Sodium Phosphate, 0.15M NaCl, pH 8.5, 15mg/mL BSA, 0.05% Azide
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Preservative
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Azide
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Working Concentration
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Information
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Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, horse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species. |
Company
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Jackson ImmunoResearch
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