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Alkaline Phosphatase-AffiniPure F(ab')2 Fragment Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs,Rb Sr Prot)

 เพิ่มเมื่อ: 2016-11-29 12:24:07.0

รายละเอียด:

  • Catalog No.JIR-112-056-143
  • Product Name Alkaline Phosphatase-AffiniPure F(ab')2 Fragment Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs,Rb Sr Prot)
  • Unit0.5 ml
  • ConjugatedAlkaline Phosphatase
  • TargetRat
  • HostGoat
  • Product TypeF(ab')? Fragment
  • SpecificityIgG (H+L)
  • ClonalityPolyclonal
  • Physical StateFreeze-dried powder
  • StorageStore freeze-dried powder at 2-8? C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol ( ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original.
  • Expiration dateone year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
  • Purity
  • Buffer0.01M Tris-HCl, 0.25M NaCl, pH 8.0, 15 mg/mL BSA, 0.05% Azide
  • PreservativeAzide
  • Working
  • Information

    F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

    Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, horse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
  • CompanyJackson ImmunoResearch
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Catalog No.
JIR-112-056-143
Product Name
Alkaline Phosphatase-AffiniPure F(ab')2 Fragment Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs,Rb Sr Prot)
Unit
0.5 ml
Conjugated
Alkaline Phosphatase
Target
Rat
Host
Goat
Product Type
F(ab')? Fragment
Specificity
IgG (H+L)
Clonality
Polyclonal
Physical State
Freeze-dried powder
Storage
Store freeze-dried powder at 2-8? C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol ( ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original.
Expiration date
one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Purity
Buffer
0.01M Tris-HCl, 0.25M NaCl, pH 8.0, 15 mg/mL BSA, 0.05% Azide
Preservative
Azide
Working Concentration
Information

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, horse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
Company
Jackson ImmunoResearch

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