F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of rat IgM but not with rat IgG or the light chains of rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with IgM from other species.
Catalog No.
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JIR-112-006-075
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Product Name
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AffiniPure F(ab')2 Fragment Goat Anti-Rat IgM, ? Chain Specific (min X Hu,Bov,Hrs Sr Prot)
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Unit
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1 mg
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Conjugated
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Unconjugated
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Target
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Rat
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Host
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Goat
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Product Type
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F(ab')? Fragment
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Specificity
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IgM, ? Chain
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Clonality
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Polyclonal
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Physical State
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Sterile-filtered liquid
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Storage
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Store at 2-8°C under sterile conditions. Prepare working dilution fresh each day.
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Expiration date
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one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
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Purity
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Buffer
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0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
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Preservative
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Working Concentration
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Information
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F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of rat IgM but not with rat IgG or the light chains of rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with IgM from other species. |
Company
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Jackson ImmunoResearch
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