Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
Catalog No.
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JIR-109-205-088
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Product Name
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12nm Colloidal Gold-AffiniPure Goat Anti-Human IgG (H+L) (min X Bov,Hrs,Ms Sr Prot) (EM Grade)
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Unit
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0.5 ml
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Conjugated
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12 nm Gold
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Target
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Human
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Host
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Goat
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Product Type
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Whole IgG
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Specificity
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IgG (H+L)
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Clonality
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Polyclonal
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Physical State
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Sterile-filtered liquid
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Storage
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Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Prepare working dilution fresh each day.
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Expiration date
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six months from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
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Purity
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Buffer
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0.01M Sodium Borate-Sodium Phosphate, 0.15M NaCl, pH 8.5, 15mg/mL BSA, ~ 50% glycerol
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Preservative
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Working Concentration
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Information
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Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species. |
Company
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Jackson ImmunoResearch
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