Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
Catalog No.
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JIR-109-135-098
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Product Name
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Allophycocyanin-AffiniPure Goat Anti-Human IgG, Fc? Fragment Specific (min X Bov,Hrs,Ms Sr Prot)
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Unit
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0.5 ml
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Conjugated
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APC
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Target
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Human
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Host
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Goat
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Product Type
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Whole IgG
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Specificity
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IgG, Fc? Fragment
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Clonality
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Polyclonal
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Physical State
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Freeze-dried powder
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Storage
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Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Store at 2-8°C - DO NOT FREEZE. Prepare working dilution fresh each day.
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Expiration date
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six months from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
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Purity
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Buffer
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0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15mg/mL BSA, 0.05% Azide
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Preservative
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Azide
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Working Concentration
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Information
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Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species. |
Company
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Jackson ImmunoResearch
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