R-Phycoerythrin-AffiniPure F(ab')2 Fragment Goat Anti-Human IgM, Fc5? Fragment Specific (min X Bov Sr Prot)

JIR-109-116-129

R-Phycoerythrin-AffiniPure F(ab')2 Fragment Goat Anti-Human IgM, Fc5? Fragment Specific (min X Bov Sr Prot)

1 ml

R-Phycoerythrin

Human

Goat

F(ab')? Fragment

IgM, Fc5? Fragment

Polyclonal

Freeze-dried powder

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Store at 2-8°C - DO NOT FREEZE. Prepare working dilution fresh each day.

six months from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15mg/mL BSA, 0.05% Azide

Azide

F(ab')₂ fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')₂ fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc_5µ portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with IgM from other species.

Jackson ImmunoResearch