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Sygnis TruePrime™ RCA Kit

 เพิ่มเมื่อ: 2016-12-21 14:27:42.0
 แก้ไขล่าสุด: 2016-12-21 14:47:05.0

รายละเอียด:
Sygnis TruePrime™ RCA Kit
  • detailA single- or double-stranded circular DNA amplification kit with less bias and more uniform amplification starting with limiting amounts of DNA or bacterial cells. - Reduced amplification bias and no primer artefacts: Primer-free rolling circle amplification (RCA) method utilizing a combination of TthPrimPol Primase (to synthesize primers) and Phi29 DNA polymerase. - Highly sensitive: Amplification of attogram amounts of purified input DNA - Easy handling and high reproducibility - Insensitive to external DNA contaminations
  • descriptionA novel multiple displacement amplification kit that combines of the recently discovered DNA primase “TthPrimPol” and the extremely processive and high-fidelity Phi29 DNA polymerase to amplify single- or double-stranded circular DNA molecules by rolling circle amplification (RCA).
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 A single- or double-stranded circular DNA amplification kit with less bias and more uniform amplification starting with limiting amounts of DNA or bacterial cells.
- Reduced amplification bias and no primer artefacts: Primer-free rolling circle amplification (RCA) method utilizing a combination of TthPrimPol Primase (to synthesize primers) and Phi29 DNA polymerase.
- Highly sensitive: Amplification of attogram amounts of purified input DNA
- Easy handling and high reproducibility
- Insensitive to external DNA contaminations

 สินค้าที่เกี่ยวข้อง

 The TruePrime™ RCA kit uses a novel multiple displacement amplification method based on the combination of the recently discovered DNA primase TthPrimPol and the highly processive and high-fidelity Phi29 DNA polymerase to amplify single- or double-stranded circular DNA molecules by rolling circle amplification (RCA). The strong strand displacement capacity of Phi29 DNA polymerase allows TthPrimPol to generate new primers on the displaced strands that are extended by Phi29 DNA pol, resulting in exponential isothermal DNA amplification. The product of TruePrime™ RCA reaction is high molecular weight, double-stranded concatemers of the circular template. The TruePrime™ RCA kit eliminates the need for overnight cell culture and conventional DNA purification. Typical DNA yields from a 25 µL reaction are >3 µg for a 3 hour reaction when starting from 1 ng of input plasmid DNA (3-10 kb). For 150-200 kb templates, yields are approximately 2 µg per 25 µL reaction with 10 ng DNA and a 3 hour reaction.

 
Suitable starting materials include:
 
Purified DNA: plasmids, cosmids, BACs, M13 clones, etc.
Bacterial cells: colonies picked from agar plates, bacterial cultures or glycerol stocks.
 

How does TruePrime™ Technology work? 

Excellent sensitivity with plasmids or BACs 

Absence of background amplification 

Plasmid amplification directly from bacterial cells 

Lower backgrounds and better yields than competitor 

 

Order Informaion

Each TruePrime™ RCA Kit contains: Buffer D, Buffer N, Reaction Buffer, dNTPs, Water, Enzyme 1 and Enzyme 2. The complete manual is available under the Manual tab. Lucigen is an authorized distributor of Sygnis products in the US.
 
The TruePrime™ RCA kit is intended for molecular biology use only and in vitro use only. This product is not intended for diagnosis, prevention or treatment of a disease in human beings or animals.
 

Technical Specifications:

Typical DNA yields from a 25 µL reaction are >3 µg from 1 ng of input plasmid DNA (3-10 kb) and a 3 hour reaction. For 150-200 kb templates yields are approximately 2 µg per 25 µl reaction with 10 ng input DNA and a 3 hour reaction. Yields and kinetics will vary if crude or un-quantified samples are amplified. Reactions without input DNA (no template controls) do not produce any amplification product during 3 hour reactions. Amplification products can be transformed after digestion with a single-site restriction enzyme and re-ligation.

Quality Control:

Each batch of TruePrime™ RCA kits is tested against predetermined specifications to ensure consistent product quality. Enzymes used in the kit have been tested separately to ensure adherence to specifications.

Shipping and Storage:

Kits are shipped on dry ice. Upon receipt, the kit should be stored immediately at -20°C in a non-frost-free (constant-temperature) freezer. When stored under these conditions and handled correctly, the products can be kept at least six months after shipping without showing any reduction in performance. For longer periods of time, store the kit at -80°C.

Manual

File Name Manual
Sygnis-Handbook_TruePrime_RCA_Kit PDF

Resource

Sygnis-TruePrime™-RCA-kit-MSDS.pdf

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