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OmniAmp® RNA & DNA LAMP Kit

 เพิ่มเมื่อ: 2016-12-20 16:13:02.0
 แก้ไขล่าสุด: 2016-12-20 18:44:19.0

รายละเอียด:
OmniAmp® RNA & DNA LAMP Kit
  • detail- Powerful native reverse transcriptase and strand displacement activities allow gene-specific LAMP and RT-LAMP at up to 72°C - Amplify from any nucleic acid template: RNA, DNA and cDNA - Faster than Bst Exo Minus - Complete kit with controls, Magnesium Sulfate and Betaine for easy optimization
  • descriptionThe only enzyme capable of performing LAMP directly from RNA or DNA. Faster amplification with the sensitivity needed in demanding applications.
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 - Powerful native reverse transcriptase and strand displacement activities allow  gene-specific LAMP and RT-LAMP at up to 72°C
 - Amplify from any nucleic acid template: RNA, DNA and cDNA 
 - Faster than Bst Exo Minus
 - Complete kit with controls, Magnesium Sulfate and Betaine for easy optimization

 สินค้าที่เกี่ยวข้อง

 The world's only enzyme for RNA LAMP and DNA LAMP

The OmniAmp®  polymerase is the only enzyme capable of Loop-mediated Isothermal Amplification (LAMP) from both RNA and DNA samples. The enzyme has a temperature optimum of 72°C, allowing increased specificity and flexibility as compared with Bst Exo Minus or two-step processes. The native reverse transcriptase activity of this enzyme means no longer adding a separate reverse transcriptase for RNA targets.

Flexibility: The enzyme comes with 10X Polymerase Buffer C, Magnesium Chloride, and Betaine. The buffer is designed to allow for a wide range of additive concentrations. Easily optimize your reaction with the instructions provided in the user manual.

Reliability: Achieve faster amplification at higher temperatures without sacrificing sensitivity. The enzyme has high target specificity and low background. Every batch is quality tested to ensure no unwanted nuclease activity will interfere with your sensitive applications.
 
Primer design: A robust LAMP reaction depends on proper design of the amplification primers. Please follow this PDF guide to properly use the PrimerExplorer software. The use of six primers is optimal; reactions are significantly slower and detection is less sensitive if only four primers are used.
 
 
Fig 1. RNA LAMP reaction 
Agarose gel image of the included RNA LAMP Control Reaction. The common “ladder” banding pattern within a HMW smear is customary of LAMP reactions. No dedicated RT step or use of additional RT enzyme was used.
 

DNA LAMP: DNA reliability you need and faster amplification

 

 

Fig. 2. Quantitation of LAMP Amplification from DNA Target

Quantitative results of LAMP amplification of E. ictaluri DNA over several orders of magnitude of target concentration. Color key: 1:10 = Red, 1:100 = Blue, 1:1,000 = Brown, 1:10,000 = Green, 1:100,000 = Pink, 1:1,000,000 = Light Blue. 1:10,000,000 dilution (yellow) and NTC's (Black) showed no amplification. The cycler was programmed to read amplification in 30-second intervals (cycles).

Instrument flexibility: DNA LAMP reaction performed using a heat block

Fig. 3. DNA LAMP amplification

Agarose gel image of a DNA LAMP reaction performed with several dilutions of E. ictaluri DNA using a heat block instead of a thermocycler. After 20 minutes the reactions were stopped by adding gel loading dye and EDTA. 

Licensing information: Lucigen is a fully licensed provider of LAMP reagents for research use. Patents WO 00/28082, WO 01/34790, and WO 01/77317 regarding the LAMP method are owned by the Eiken Chemical Co. Ltd. OmniAmp™ and Bst Polymerase, Exonuclease minus are sold by Lucigen under license for use in LAMP for research use only. The products may not be used for LAMP-based human or diagnostic purposes without obtaining a license from Eiken. US Patent 8093030 for OmniAmp DNA Polymerase is owned by Lucigen Corp. 

It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties. If the purchaser is not willing to accept these use limitations, Lucigen Corporation is willing to accept return of the product for a full refund. 

Interested in OEM Opportunities for OmniAmp™ or other Lucigen products? Please contact us at Lucigen@lucigen.com.

 ORDER INFORMATION

OmniAmp® RNA & DNA LAMP Kit is provided as a 50X enzyme with 10X DNA Polymerase Buffer C, 100 mM MgSO4, 5M Betaine, Nuclease-free water, a control RNA template and control LAMP reaction primers. Standard reaction is 25 uL.

The enzyme is provided in a storage buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, and 50% Glycerol. 10X DNA Polymerase Buffer C is composed of 200 mM Tris-HCl pH 8.0, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4,  and 1.0 % Triton X-100.

Specifications

Absence of Endonuclease or Nicking Activity: Incubation of OmniAmp™ DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70°C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis.

Absence of Exonuclease Activity: Incubation of of OmniAmp DNA Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 70°C resulted in no smearing of bands on agarose gels.

Purity: OmniAmp DNA Polymerase is >99% pure as determined by SDS-PAGE.

Exogenous DNA: There is no detectable DNA contamination.

Manuals

Document File Name Type
Manual MA148-OmniAmp-RNA-DNA-LAMP-Kit PDF
Manual Design-of-LAMP-primers-11-2015 PDF

 

 

 

Resources

Document File Name Type
Poster OmniAmp-AMP-Mtg-Nov-2013 PDF
Product Flyer F202-US032016-OmniAmp PDF
SDS SDS014 - 30065-1_30065-2 OmniAmp RNA and DNA Master Mix.pdf PDF

 

 

 

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