Support
Pacific Science
Your shopping cart
ดูตะกร้าสินค้าของคุณ
ไม่มีสินค้าในตะกร้าของคุณ
จำนวนครั้งที่เปิดดูสินค้า : 278 | ความคิดเห็น: 0

ReverTra Ace® qPCR RT Master Mix with gDNA Remover

 เพิ่มเมื่อ: 2016-12-23 14:16:36.0

รายละเอียด:
High efficient cDNA synthesis master mix for real-time PCR with gDNA remover
  • Catalog No.TYB-FSQ-301   
  • DescriptionThis kit includes reagents for reverse transcription and for the removal of genomic DNA [DNase I treatment]. In many cases, total RNA prepared using spin-columns or acid guanidium-phenolchloroform (AGPC) extraction methods contains small amount of genomic DNA. Any contaminating genomic DNA will be amplified along with cDNA, especially when primer pairs are designed within the same exon or from pseudogenes. Amplification from genomic DNA can result in qualitative and quantitative inaccuracies. The protocol consists of (i) a genomic DNA degradation step using "gDNA remover" and (ii) a reverse transcription step. The two steps can be achieved sequentially without purification or heat inactivation of DNase I.
  • Feature1 "Genomic DNA degradation step" and "cDNA synthesis step" can be achieved sequentially in approximately 30 min. 2 The master mix reagent contains random and oligo dT primers optimized for efficient reverse transcription. 3 Control, no reverse transcription experiments (no RT-control) can be performed with 5x RT Master Mix II no-RT control.
  • Volumn Unit200 reactions
  • ApplicationHigh efficient cDNA synthesis master mix for real-time PCR with gDNA remover
  • CompanyTOYOBO
  • ManualReverTra Ace® qPCR RT Master Mix with gDNA Remover
ไม่ระบุราคา
Product
ReverTra Ace® qPCR RT Master Mix with gDNA Remover
Catalog No.
TYB-FSQ-301   
Description
This kit includes reagents for reverse transcription and for the removal of genomic DNA [DNase I treatment]. In many cases, total RNA prepared using spin-columns or acid guanidium-phenolchloroform (AGPC) extraction methods contains small amount of genomic DNA. Any contaminating genomic DNA will be amplified along with cDNA, especially when primer pairs are designed within the same exon or from pseudogenes. Amplification from genomic DNA can result in qualitative and quantitative inaccuracies. The protocol consists of (i) a genomic DNA degradation step using "gDNA remover" and (ii) a reverse transcription step. The two steps can be achieved sequentially without purification or heat inactivation of DNase I.
Feature
1 "Genomic DNA degradation step" and "cDNA synthesis step" can be achieved sequentially in approximately 30 min.
2 The master mix reagent contains random and oligo dT primers optimized for efficient reverse transcription.
3 Control, no reverse transcription experiments (no RT-control) can be performed with 5x RT Master Mix II no-RT control.
Volumn Unit and Reaction
200 reactions
Application
High efficient cDNA synthesis master mix for real-time PCR with gDNA remover
Company
TOYOBO
Manual

 สินค้าที่เกี่ยวข้อง

guest
ชื่อ
Email
เบอร์โทรศัพท์