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KOD -Plus- Neo

 เพิ่มเมื่อ: 2016-12-23 14:07:37.0

รายละเอียด:
High fidelity PCR Fast and efficient PCR
  • Catalog No.TYB-KOD-401
  • DescriptionKOD -Plus- Neo is based on a DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1(1) (2). This polymerase exhibits excellent PCR fidelity because of its efficient 3'→5' exonuclease activity (proof reading activity). This product contains a unique "elongation enhancer" that suppresses the "plateau effect" produced by conventional PCR. Therefore, this reagent exhibits greater amplification efficiency and elongation capability compared to the previous version of KOD -Plus- (Code No. KOD-201). Moreover, this enzyme requires only 30 sec/kb for the PCR extension step. This facilitates the long range PCR. This enzyme contains two anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR(3). This polymerase generates blunt-end PCR products due to 3'→5' exonuclease (proof-reading) activity.
  • FeatureKOD -Plus- Neo exhibits 80-fold greater PCR fidelity than Taq DNA polymerase. 2 "Elongation enhancer" enables greater 3 amplification efficiency and elongation capability (up to 24 kb from human genomic DNA) compared to conventional PCR. 4 Requires only 30 sec/kb for the PCR extension step. 5 2-step cycle conditions can be used for amplification using ≥ 20 mer primers (melting temperatures, Tm >63ºC).
  • Volumn Unit200 U / 200 reactions
  • ApplicationHigh fidelity PCR Fast and efficient PCR
  • CompanyTOYOBO
  • ManualKOD -Plus- Neo
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Product
KOD -Plus- Neo
Catalog No.
TYB-KOD-401
Description
KOD -Plus- Neo is based on a DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1(1) (2). This polymerase exhibits excellent PCR fidelity because of its efficient 3'→5' exonuclease activity (proof reading activity). This product contains a unique "elongation enhancer" that suppresses the "plateau effect" produced by conventional PCR. Therefore, this reagent exhibits greater amplification efficiency and elongation capability compared to the previous version of KOD -Plus- (Code No. KOD-201). Moreover, this enzyme requires only 30 sec/kb for the PCR extension step. This facilitates the long range PCR. This enzyme contains two anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR(3). This polymerase generates blunt-end PCR products due to 3'→5' exonuclease (proof-reading) activity.
Feature
KOD -Plus- Neo exhibits 80-fold greater PCR fidelity than Taq DNA polymerase.
2 "Elongation enhancer" enables greater 
3 amplification efficiency and elongation capability (up to 24 kb from human genomic DNA) compared to conventional PCR.
4 Requires only 30 sec/kb for the PCR extension step.
5 2-step cycle conditions can be used for amplification using ≥ 20 mer primers (melting temperatures, Tm >63ºC).
Volumn Unit and Reaction
200 U / 200 reactions 
Application
High fidelity PCR Fast and efficient PCR
Company
TOYOBO
Manual

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